Services Available

We offer personalized project advice and planning.  All technologies include an initial consultation to discuss experimental design and procedures.

Next Generation Sequencing Library Preparation

We provide library preparation services for a wide range of applications:

Services Provided:

  • PCR-free
  • Amplified
  • Formalin-fixed paraffin-embedded (FFPE)

Services Provided:

  • Single Cell RNA Sequencing (scRNAseq)
  • Ribodepleted, Strand Specific RNA Sequencing 
  • RNA Quality Testing (Agilent Bioanalyzer 2100)

Available Technologies:

  • Native Chromatin Immunoprecipitation Sequencing (ndChIP-Seq) for the following histone marks:
    • H3K4me1
    • H3K4me3
    • H3K9me3
    • H3K27me3
    • H3K27ac
    • H3K36me2
    • H3K36me3
    • H2AK119ub1
  • See below for the Cell preparation SOP
EGL031.03_Sample preparation for epigenomic profiling using native Chip-seq[1][1][2][1]

Available WGBS Technologies:

  • Post-Bisulfite Adapter Ligation (PBAL)
  • Biomodal 5-base
  • Biomodal 6-base
  • Illumina 5-base

Available Technologies:

  • Micro-C sequencing (Dovetail Genomics)

Available Technologies:

  • ATAC-seq library preparation (Diagenode)

Available Technologies

  • Two step PCR strategy for generation of indexed amplicon libraries ready for NGS

  • Single cell methylation profiling (10X Genomics)
  • Single cell methylation profiling scPBAL (96 well format)
  • Single cell transcriptome (Universal 3’/5’ and Flex) (10X Genomics)
  • Single cell Epi Multiome (ATAC + Gene expression) (10X Genomics)
  • Single cell ATAC Epi Chromatin (10X Genomics)

Next Generation Sequencing

With the latest Illumina sequencing system NovaSeq X, we offer maximum throughput and accuracy with extraordinary sequencing power for data-intensive applications.

  • 10B (PE150) FC Available: ~1.5B Paired Reads per Lane
  • 25B (PE150) FC Available: ~4B Paired Reads per Lane
  • Sequencing libraries should be diluted in 10mM Tris-HCl pH 8 buffer and must not contain chelating agent (e.g., EDTA).
  • Submission requirements:  30uL of 5nM for each NovaSeq X Lane.

Contact us for up to date pricing.

For NextSeq2000 sequencing please contact SMBE-seq directly:

https://bme.ubc.ca/home/sequencing-facility/

Learn more about NovaSeqX
Learn more about NextSeq2000

FASTQ files are the usual deliverables for the Illumina sequencing, but it is possible to generate BAM files for Human, Mouse and Rat species. The sequencing data can be downloaded directly from Illumina cloud (ICA). Click here to learn more about ICA.

Other Services

We offer library QC and sequencing services for Illumina-compatible user-prepared libraries.

  • We offer genomic DNA and RNA extraction from both tissue and frozen cell pellets (human/mouse)

  • For user generated sequencing pools, we offer sequencing pool quantification (qPCR and fragment analysis) to accurately determine clustering capacity for optimal loading

Available Technologies:

  • Qubit Fluorometric Quantification
  • Agilent Bioanalyzer 2000
  • Quantitative PCR